bbsi entry vector (Addgene inc)
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95
Structured Review
Addgene inc
bbsi entry vector
Bbsi Entry Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbsi+entry+vector/pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W+(Plasmid+%2367974)/bio_rxiv__2025__04__15__648737-195-49-52
Average 95 stars, based on 119 article reviews
Bbsi Entry Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbsi+entry+vector/pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W+(Plasmid+%2367974)/bio_rxiv__2025__04__15__648737-195-49-52
Average 95 stars, based on 119 article reviews
bbsi entry vector - by Bioz Stars,
2026-10
95/100 stars
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Transformation Assay:Article Title: CRISPR screens in the context of immune selection identify CHD1 and MAP3K7 as mediators of cancer immunotherapy resistance Article Snippet: Individual gRNAs sequences were extracted from MinLib gRNAs (Addgene #164896), ordered as oligos (Sigma-Aldrich), and cloned using Golden Gate cloning. .. Our procedure made use of primers encoding a gRNA with BbsI overhangs and an additional G for hU6 RNApolIII transcription (Forward: 5ʹ-CACCGNNNNNNNNNNNNNNNNNNN-3ʹ and Reverse: 5ʹ-AAACNNNNNNNNNNNNNNNNNNC-3ʹ), annealed by boiling (100°C 5 mins) and slowly cooling to room temperature (0.1°C per second until reaching 25°C), and then ligated duplexes with a |